Click here for three peer-reviewed protocols that you can try in your own In single-cell adult cardiomyocyte experiments, isolation is the most.
All research on mice was done according to procedures and guidelines of the National Institutes of Health, and the protocols were.
J Vis Exp. Aug 28;(). doi: / Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes. Judd J(1), Lovas J(1), Huang GN( 2).
Current protocols rely on retrograde aortic perfusion using specialized Langendorff Isolation and culture of adult mouse cardiac myocytes.